The Impact of Ice Compression and Massage Techniques on Lactate Removal and Interleukin-6 Concentrations During Halftime Recovery in Intermittent Sports
DOI:
https://doi.org/10.17309/tmfv.2025.5.01Keywords:
ice compression, sport massage, lactate clearance, interleukin-6, inflammatory responseAbstract
Background. High-intensity intermittent sports increase blood lactate levels and inflammatory markers such as interleukin-6 (IL-6), which may hinder recovery and performance. Effective recovery strategies are crucial for enhancing lactate clearance and regulating inflammation.
Objectives. This study aimed to evaluate the effects of 15-minute ice compression and sport massage interventions during halftime on athletes’ lactate clearance and IL-6 levels.
Materials and Methods. Thirty-six male soccer players, aged 18 to 24 years, were randomly assigned to one of three groups for this experimental study: ice compression (IC, n = 12), sport massage (SM, n = 12), and passive recovery (PR, n = 12). Each participant completed a standardized exercise regimen designed to induce fatigue. The PR group rested passively after the workout, while the intervention groups received a massage or ice compression for 15 minutes. Blood lactate and IL-6 levels were assessed before exercise (T0), immediately after (T1), and fifteen minutes post-exercise (T2). To analyse the differences observed, a mixed-model ANOVA and post-hoc tests (Bonferroni/Games-Howell) were performed.
Results. According to the results obtained, the lactate levels (IC: ΔT1-T2 = -2.34 ± 0.96 mmol/L, d = 2.209; SM: ΔT1-T2 = -1.39 ± 0.79 mmol/L, d = 0.829) and IL-6 levels (IC: ΔT1-T2 = -1.44 ± 0.93 pg/mL, d = 1.206; SM: ΔT1-T2 = -1.58 ± 0.63 pg/mL, d = 1.645) were both significantly lower than PR (p < 0.05). Although IC and SM exhibited similar anti-inflammatory effects (p = 0.898), IC demonstrated superior lactate clearance compared to SM (p = 0.023, d = -1.081). PR showed only minimal decreases in both biomarkers.
Conclusions. Massage and ice compression effectively enhance lactate clearance after high-intensity exercise, with massage offering additional benefits in reducing IL-6-mediated inflammation. These findings support massage as an excellent recovery method for athletes requiring immediate physiological repair following exertion.
Downloads
References
Marqués-Jiménez, D., Calleja-González, J., Arratibel, I., Delextrat, A., & Terrados, N. (2017). Fatigue and Recovery in Soccer: Evidence and Challenges. The Open Sports Sciences Journal, 10(1), 52-70. https://doi.org/10.2174/1875399X01710010052 DOI: https://doi.org/10.2174/1875399X01710010052
Reilly, T., Drust, B., & Clarke, N. (2008). Muscle fatigue during football match-play. Sports Medicine, 38(5), 357-367. https://doi.org/10.2165/00007256-200838050-00001 DOI: https://doi.org/10.2165/00007256-200838050-00001
Chen, G., Liu, J., Guo, Y., & Sun, P. (2025). Mechanisms for Regulatory Effects of Exercise on Metabolic Diseases from the Lactate–Lactylation Perspective. International Journal of Molecular Sciences, 26(8), 3469. https://doi.org/10.3390/IJMS26083469 DOI: https://doi.org/10.3390/ijms26083469
Wan, J. J., Qin, Z., Wang, P. Y., Sun, Y., & Liu, X. (2017). Muscle fatigue: general understanding and treatment. Experimental & Molecular Medicine, 49(10), e384. https://doi.org/10.1038/EMM.2017.194 DOI: https://doi.org/10.1038/emm.2017.194
Vavřička, J., Brož, P., Follprecht, D., Novák, J., & Kroužecký, A. (2024). Modern Perspective of Lactate Metabolism. Physiological Research, 73(4), 499-514. https://doi.org/10.33549/physiolres.935331 DOI: https://doi.org/10.33549/physiolres.935331
Cairns, S. P. (2006). Lactic acid and exercise performance: Culprit or friend? Sports Medicine, 36(4), 279-291. https://doi.org/10.2165/00007256-200636040-00001 DOI: https://doi.org/10.2165/00007256-200636040-00001
Lin, W., Song, H., Shen, J., Wang, J., Yang, Y., Yang, Y., Cao, J., Xue, L., Zhao, F., Xiao, T., & Lin, R. (2023). Functional role of skeletal muscle-derived interleukin-6 and its effects on lipid metabolism. Frontiers in Physiology, 14, 1110926. https://doi.org/10.3389/FPHYS.2023.1110926/XML/NLM DOI: https://doi.org/10.3389/fphys.2023.1110926
Covarrubias, A. J., & Horng, T. (2014). IL-6 strikes a balance in metabolic inflammation. Cell Metabolism, 19(6), 898. https://doi.org/10.1016/J.CMET.2014.05.009 DOI: https://doi.org/10.1016/j.cmet.2014.05.009
Nash, D., Hughes, M. G., Butcher, L., Aicheler, R., Smith, P., Cullen, T., & Webb, R. (2023). IL-6 signaling in acute exercise and chronic training: Potential consequences for health and athletic performance. Scandinavian Journal of Medicine and Science in Sports, 33(1), 4-19. https://doi.org/10.1111/sms.14241 DOI: https://doi.org/10.1111/sms.14241
Fajar, M. K., Hariyanto, A., Wahjuni, E. S., Kartiko, D. C., Siantoro, G., Phanpheng, Y., Kusuma, I. D. M. A. W., Pramono, B. A., Rusdiawan, A., & Pranoto, A. (2025). Determining the Efficacy of Providing Ice Compression for Fitness Enhancement After Speed Endurance Exercise on Reducing Heart Rate, IL-6, and Fatigue Index in Football Athletes. Physical Education Theory and Methodology, 25(1), 58-65. https://doi.org/10.17309/tmfv.2025.1.07 DOI: https://doi.org/10.17309/tmfv.2025.1.07
Romagnoli, M., Sanchis-Gomar, F., Alis, R., Risso-Ballester, J., Bosio, A., Graziani, R. L., & Rampinini, E. (2016). Changes in muscle damage, inflammation, and fatigue-related parameters in young elite soccer players after a match. The Journal of Sports Medicine and Physical Fitness, 56(10), 1198-1205.
Kafrawi, F. R., Nurhasan, Wahjuni, E. S., Rusdiawan, A., Bekti, A. P., & Ayubi, N. (2024). Sports massage has the potential to reduce ischemic muscle pain and increase range of motion after exercise. Fizjoterapia Polska, 2024(1), 60-65. https://doi.org/10.56984/8ZG2EF85A3 DOI: https://doi.org/10.56984/8ZG2EF85A3
White, G. E., & Wells, G. D. (2013). Cold-water immersion and other forms of cryotherapy: physiological changes potentially affecting recovery from high-intensity exercise. Extreme Physiology & Medicine, 2(1), 26. https://doi.org/10.1186/2046-7648-2-26 DOI: https://doi.org/10.1186/2046-7648-2-26
Yao, Y., Xie, W., Opoku, M., Vithran, D. T. A., Li, Z., & Li, Y. (2024). Cryotherapy and thermotherapy in the management of osteoarthritis and rheumatoid arthritis: A comprehensive review. Fundamental Research. https://doi.org/10.1016/J.FMRE.2024.07.008 DOI: https://doi.org/10.1016/j.fmre.2024.07.008
Peake, J. M., Roberts, L. A., Figueiredo, V. C., Egner, I., Krog, S., Aas, S. N., Suzuki, K., Markworth, J. F., Coombes, J. S., Cameron-Smith, D., & Raastad, T. (2016). The effects of cold water immersion and active recovery on inflammation and cell stress responses in human skeletal muscle after resistance exercise. The Journal of Physiology, 595(3), 695. https://doi.org/10.1113/JP272881 DOI: https://doi.org/10.1113/JP272881
Peake, J. M., Markworth, J. F., Nosaka, K., Raastad, T., Wadley, G. D., & Coffey, V. G. (2015). Modulating exercise-induced hormesis: Does less equal more? Journal of Applied Physiology, 119(3), 172-189. https://doi.org/10.1152/japplphysiol.01055.2014 DOI: https://doi.org/10.1152/japplphysiol.01055.2014
Mila-Kierzenkowska, C., Jurecka, A., Woźniak, A., Szpinda, M., Augustyńska, B., & Woźniak, B. (2013). The effect of submaximal exercise preceded by single whole-body cryotherapy on the markers of oxidative stress and inflammation in blood of volleyball players. Oxidative Medicine and Cellular Longevity. https://doi.org/10.1155/2013/409567 DOI: https://doi.org/10.1155/2013/409567
Crane, J. D., Ogborn, D. I., Cupido, C., Melov, S., Hubbard, A., Bourgeois, J. M., & Tarnopolsky, M. A. (2012). Massage therapy attenuates inflammatory signaling after exercise-induced muscle damage. Science Translational Medicine, 4(119). https://doi.org/10.1126/SCITRANSLMED.3002882 DOI: https://doi.org/10.1126/scitranslmed.3002882
Ogai, R., Yamane, M., Matsumoto, T., & Kosaka, M. (2008). Effects of petrissage massage on fatigue and exercise performance following intensive cycle pedalling. British Journal of Sports Medicine, 42(10), 534-538. https://doi.org/10.1136/BJSM.2007.044396 DOI: https://doi.org/10.1136/bjsm.2007.044396
Wiltshire, E. V., Poitras, V., Pak, M., Hong, T., Rayner, J., & Tschakovsky, M. E. (2010a). Massage impairs postexercise muscle blood flow and “lactic acid” removal. Medicine and Science in Sports and Exercise, 42(6), 1062-1071. https://doi.org/10.1249/MSS.0B013E3181C9214F DOI: https://doi.org/10.1249/MSS.0b013e3181c9214f
Waters-Banker, C., Dupont-Versteegden, E. E., Kitzman, P. H., & Butterfield, T. A. (2014). Investigating the Mechanisms of Massage Efficacy: The Role of Mechanical Immunomodulation. Journal of Athletic Training, 49(2), 266. https://doi.org/10.4085/1062-6050-49.2.25 DOI: https://doi.org/10.4085/1062-6050-49.2.25
Wiltshire, E. V., Poitras, V., Pak, M., Hong, T., Rayner, J., & Tschakovsky, M. E. (2010b). Massage impairs postexercise muscle blood flow and “lactic Acid” removal. Medicine and Science in Sports and Exercise, 42(6), 1062-1071. https://doi.org/10.1249/MSS.0B013E3181C9214F DOI: https://doi.org/10.1249/MSS.0b013e3181c9214f
Sut, N., Kocak, Z., Korkmaz, S., & Uzun, C. (2022). Compliance of abstracts of randomized control trials with CONSORT guidelines: A case study of Balkan journals. European Science Editing, 48(July 2021), 1-10. https://doi.org/10.3897/ese.2022.e71240 DOI: https://doi.org/10.3897/ese.2022.e71240
Li, H., Early, K. S., Zhang, G., Ma, P., & Wang, H. (2024). Personalized Hydration Strategy to Improve Fluid Balance and Intermittent Exercise Performance in the Heat. Nutrients, 16(9), 1341. https://doi.org/10.3390/NU16091341 DOI: https://doi.org/10.3390/nu16091341
Lateef, F. (2010). Post exercise ice water immersion: Is it a form of active recovery? Journal of Emergencies, Trauma and Shock, 3(3), 302. https://doi.org/10.4103/0974-2700.66570 DOI: https://doi.org/10.4103/0974-2700.66570
VanderVeen, B. N., Fix, D. K., Montalvo, R. N., Counts, B. R., Smuder, A. J., Murphy, E. A., Koh, H. jin, & Carson, J. A. (2019). The Regulation of Skeletal Muscle Fatigability and Mitochondrial Function by Chronically Elevated IL-6. Experimental Physiology, 104(3), 385. https://doi.org/10.1113/EP087429 DOI: https://doi.org/10.1113/EP087429
He, J., Zhang, X., Ge, Z., Shi, J., Guo, S., & Chen, J. (2025). Whole-body cryotherapy can reduce the inflammatory response in humans: a meta-analysis based on 11 randomized controlled trials. Scientific Reports, 15(1). https://doi.org/10.1038/S41598-025-90396-3 DOI: https://doi.org/10.1038/s41598-025-90396-3
Khoshnevis, S., Craik, N. K., & Diller, K. R. (2014). Cold-induced vasoconstriction may persist long after cooling ends: an evaluation of multiple cryotherapy units. Knee Surgery, Sports Traumatology, Arthroscopy: Official Journal of the ESSKA, 23(9), 2475. https://doi.org/10.1007/S00167-014-2911-Y DOI: https://doi.org/10.1007/s00167-014-2911-y
Hyldahl, R. D., & Peake, J. M. (2020). Combining cooling or heating applications with exercise training to enhance performance and muscle adaptations. Journal of Applied Physiology, 129(2), 353-365. https://doi.org/10.1152/JAPPLPHYSIOL.00322.2020 DOI: https://doi.org/10.1152/japplphysiol.00322.2020
Weerapong, P., Hume, P. A., & Kolt, G. S. (2005). The mechanisms of massage and effects on performance, muscle recovery and injury prevention. Sports Medicine, 35(3), 235-256. https://doi.org/10.2165/00007256-200535030-00004 DOI: https://doi.org/10.2165/00007256-200535030-00004
Aragón-Vela, J., Fontana, L., Casuso, R. A., Plaza-Díaz, J., & R. Huertas, J. (2021). Differential inflammatory response of men and women subjected to an acute resistance exercise. Biomedical Journal, 44(3), 338. https://doi.org/10.1016/J.BJ.2020.02.005 DOI: https://doi.org/10.1016/j.bj.2020.02.005
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2025 Afif Rusdiawan, Achmad Widodo, Ahmad Lani, Cemal Özman, Ardo Yulpiko Putra, Nugroho Susanto, Rizky Aris Munandar, Muhammad Firdaus Kafrawi, Farizha Irmawati

This work is licensed under a Creative Commons Attribution 4.0 International License.
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).

